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unconjugated sambucus nigra lectin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories unconjugated sambucus nigra lectin
    Unconjugated Sambucus Nigra Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 599 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/L-1300-5/Unconjugated+Sambucus+Nigra+Lectin+(SNA%2C+EBL)/10__2147_slash_ijn__s630472-32-0-17
    Average 95 stars, based on 599 article reviews
    unconjugated sambucus nigra lectin - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Immunopeptidomics:

    Article Title: Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRP αV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197- H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa Fluor 488 (kindly provided by Carolyn Shurer), MHC class I H-2Db presenting the LCMV-derived gp33 peptide (KAVYNFATM) [ 37, 38 ], MHC class I (HLA-A*02:01) presenting the tumor-associated antigen NY-ESO1-9 V peptide (SLLMWITQV; from now on 9 V) [ 39 ], MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR [ 38 ], 1G4 TCR [ 39 ], Wheat Germ Agglutinin (WGA) Alexa Fluor 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa Fluor 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labeling.

    Article Title: Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRPαV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197-H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa FluorTM 488 (kindly provided by Carolyn Shurer), MHC class I H-2D b presenting the LCMV-derived gp33 peptide (KAVYNFATM) ( ; ), MHC class I (HLA-A*02:01) presenting the tumour-associated antigen NY-ESO1-9V peptide (SLLMWITQV; from now on 9V) , MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR , 1G4 TCR , Wheat Germ Agglutinin (WGA) Alexa FluorTM 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa FluorTM 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labelling.

    Variant Assay:

    Article Title: Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRP αV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197- H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa Fluor 488 (kindly provided by Carolyn Shurer), MHC class I H-2Db presenting the LCMV-derived gp33 peptide (KAVYNFATM) [ 37, 38 ], MHC class I (HLA-A*02:01) presenting the tumor-associated antigen NY-ESO1-9 V peptide (SLLMWITQV; from now on 9 V) [ 39 ], MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR [ 38 ], 1G4 TCR [ 39 ], Wheat Germ Agglutinin (WGA) Alexa Fluor 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa Fluor 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labeling.

    Article Title: Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRPαV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197-H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa FluorTM 488 (kindly provided by Carolyn Shurer), MHC class I H-2D b presenting the LCMV-derived gp33 peptide (KAVYNFATM) ( ; ), MHC class I (HLA-A*02:01) presenting the tumour-associated antigen NY-ESO1-9V peptide (SLLMWITQV; from now on 9V) , MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR , 1G4 TCR , Wheat Germ Agglutinin (WGA) Alexa FluorTM 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa FluorTM 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labelling.

    Whole Genome Amplification:

    Article Title: Studying Macromolecular Composition in Cell-Cell Interfaces Using 3D Membrane Reconstitution Systems.
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRP αV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197- H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa Fluor 488 (kindly provided by Carolyn Shurer), MHC class I H-2Db presenting the LCMV-derived gp33 peptide (KAVYNFATM) [ 37, 38 ], MHC class I (HLA-A*02:01) presenting the tumor-associated antigen NY-ESO1-9 V peptide (SLLMWITQV; from now on 9 V) [ 39 ], MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR [ 38 ], 1G4 TCR [ 39 ], Wheat Germ Agglutinin (WGA) Alexa Fluor 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa Fluor 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labeling.

    Article Title: Studying macromolecular composition in cell-cell interfaces using 3D membrane reconstitution systems
    Article Snippet: We used the following recombinant proteins obtained from Sino Biological: human CD2 (His-Tag,10982-H08H), CD58/ human LFA-3 (His-Tag, 12409-H08H), PD1/human PDCD1 (His-Tag,10377-H08H), PD-L1/human B7-H1/ CD274 (His-Tag, 10084-H08H), CD80/human B7-1 (His-Tag, 10698-H08H), human CTLA-4 (His-Tag,11159-H08H), NCR3/human NKp30 (His-Tag, 10480-H08H), human B7-H6 (His-Tag, 16140-H08H), human SLAMF6/human Ly108 (His-Tag, 11945-H08H), human CD84 (His-Tag, 10100-H08H), human SIRPαV2 (His-Tag, 30014-H08H), human CD47 (His-Tag, 12283-H08H), CD45 (ECD, His-Tag, 14197-H08H). .. We also used the proteins: CD43/ human leukosialin (His-Tag, CD3-H52H9, ACROBiosystems), MUC1-Alexa FluorTM 488 (kindly provided by Carolyn Shurer), MHC class I H-2D b presenting the LCMV-derived gp33 peptide (KAVYNFATM) ( ; ), MHC class I (HLA-A*02:01) presenting the tumour-associated antigen NY-ESO1-9V peptide (SLLMWITQV; from now on 9V) , MHC class I (HLA-A*02:01) presenting the NY-ESO1-3P9V peptide variant (SLPMWITQV; from now on 3P9V), P14 TCR , 1G4 TCR , Wheat Germ Agglutinin (WGA) Alexa FluorTM 647 Conjugate (ThermoFisherScientific), Aleuria Aurantia Lectin (AAL) (L-1390-2, Vector Labs), Sambucus Nigra-I-Agglutinin (SNA) (L-1300-5, Vector Labs), Maackia Amurensis-II Lectin (MAL-II) (L-1260-2, Vector Labs). .. We used Alexa FluorTM 488 or 647 NHS Ester (Succinimidyl Ester) (ThermoFisher Scientific) for protein labelling.

    other:

    Article Title: Glycan shielding enables TCR-sufficient allogeneic CAR-T therapy.
    Article Snippet: Article Glycan shielding enables TCR-sufficient allogeneic

    Article Title: Sialic Acid-Targeting Lectin-Conjugated Oxygen Nanosomes Alleviate Hypoxia and Promote Macrophage Activation in Pancreatic Cancer Cells
    Article Snippet: Unconjugated Sambucus nigra lectin (SNA and EBL) and Maackia amurensis lectin II (MAL II) were purchased from Vector Laboratories Inc. (Newark, CA, USA).

    Produced:

    Article Title: Cell-Based Sialoglycan Arrays for directly comparing Influenza A virus Receptor Requirements for binding and infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat-anti-human IgG ThermoFisher A-11013 Anti-HA antibody MEDI produced in HEK293F cells This paper N/A Bacterial and virus strains A/Bilthoven/16190/68 (H3N2; HK68Hu− H3) Koel et al.40 N/A A/Bilthoven/21793/72 (H3N2; EN72 Hu− H3) Koel et al.40 N/A A/Bilthoven/1761/76 (H3N2; VI75 Hu− H3) Koel et al.40 N/A A/Bilthoven/2271/76 (H3N2; TX77 Hu− H3) Koel et al.40 N/A A/Netherlands/233/82-193N (H3N2; BK79 Hu− H3) Koel et al.40 N/A A/Netherlands/620/89 (H3N2; SI87 Hu− H3) Koel et al.40 N/A A/Netherlands/179/93 (H3N2; BE92 Hu− H3) Koel et al.40 N/A A/Netherlands/178/95-145K (H3N2; WU95 Hu− H3) Koel et al.40 N/A A/Netherlands/427/98 (H3N2; SY97 Hu− H3) Koel et al.40 N/A A/Netherlands/213/03 (H3N2; FU02 Hu− H3) Koel et al.40 N/A A/Perth/16/2009 (H3N2; PE09 Hu− H3) Koel et al.40 N/A A/duck/Hunan/795/2002(H5N1; HU02 Av− H5) Liu et al.17 N/A A/Netherlands/602/2009 (H1N1; NE09Hu− H1) Maines et al.57 N/A pRluc-53CB3/T7 van der Schaar et al.58 N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 transfection reagent InvitrogenTM 11668019 Lipofectamine 3000 transfection reagent InvitrogenTM L3000150 FuGENE 6 transfection reagent Promega E2691 Maackia Amurensis Lectin I (MAL I) Vector Laboratories B-1315-2 Maackia Amurensis Lectin II (MAL II) Vector Laboratories B-1265-1 Erythrina Cristagalli Lectin (ECA) Vector Laboratories B-1145-5 Biotinylated Sambucus Nigra Lectin (SNA) Vector Laboratories B-1305-2 Sambucus Nigra Lectin (SNA) Vector Laboratories L-1300-5 Biotinylated Pan-specific Lectenz LectenzBio SK0502B Biotinylated α2,3-specific Lectenz LectenzBio SK2301B Dulbecco’s PBS + Ca2+/Mg2+ Capricorn scientific PBS-2A DMEM High Glucose with L-Glutamine Capricorn scientific CA DMEM-HA Opti-MEM + GlutaMAX GIBCO 51985–026 Penicillin-Streptomycin Thermo Fisher Scientific 15140122 Biotinylated LAMP1 (lysosomal membrane protein 1) Liu et al.17 N/A Biotinylated GPa (glycophorin A) Liu et al.13 N/A Fetal Bovine Serum Biowest S1520 Alexa Fluor647-conjugated streptavidin Invitrogen S21374 GFP-tagged CAS9PKBS Addgene Plasmid #68371 Oseltamivir Carboxylate Hoffman-La Roche Contact Roche SA sensors biolayer interferometry Sartorius 18–5020 Critical commercial assays Renilla luciferase assay system Promega E2810 (Continued on next page) iScience 28, 112549, June 20, 2025 e1 .. The human H3N2 recombinant influenza strains used in this paper represent the antigenic clusters identified since 1968 and were generated and described previously.40 They are 2:6 reassortants containing the 6 internal gene segments of strain A/Puerto Rico/ 8/1934(H1N1) and the HA and NA gene segments of: A/Bilthoven/16190/68 (HK68Hu− H3), A/Bilthoven/21793/72 (EN72Hu− H3), A/Bilthoven/1761/76 (VI75Hu− H3), A/Bilthoven/2271/76 (TX77Hu− H3), A/Netherlands/233/82-193N (BK79Hu− H3), A/Netherlands/620/89

    Virus:

    Article Title: Cell-Based Sialoglycan Arrays for directly comparing Influenza A virus Receptor Requirements for binding and infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat-anti-human IgG ThermoFisher A-11013 Anti-HA antibody MEDI produced in HEK293F cells This paper N/A Bacterial and virus strains A/Bilthoven/16190/68 (H3N2; HK68Hu− H3) Koel et al.40 N/A A/Bilthoven/21793/72 (H3N2; EN72 Hu− H3) Koel et al.40 N/A A/Bilthoven/1761/76 (H3N2; VI75 Hu− H3) Koel et al.40 N/A A/Bilthoven/2271/76 (H3N2; TX77 Hu− H3) Koel et al.40 N/A A/Netherlands/233/82-193N (H3N2; BK79 Hu− H3) Koel et al.40 N/A A/Netherlands/620/89 (H3N2; SI87 Hu− H3) Koel et al.40 N/A A/Netherlands/179/93 (H3N2; BE92 Hu− H3) Koel et al.40 N/A A/Netherlands/178/95-145K (H3N2; WU95 Hu− H3) Koel et al.40 N/A A/Netherlands/427/98 (H3N2; SY97 Hu− H3) Koel et al.40 N/A A/Netherlands/213/03 (H3N2; FU02 Hu− H3) Koel et al.40 N/A A/Perth/16/2009 (H3N2; PE09 Hu− H3) Koel et al.40 N/A A/duck/Hunan/795/2002(H5N1; HU02 Av− H5) Liu et al.17 N/A A/Netherlands/602/2009 (H1N1; NE09Hu− H1) Maines et al.57 N/A pRluc-53CB3/T7 van der Schaar et al.58 N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 transfection reagent InvitrogenTM 11668019 Lipofectamine 3000 transfection reagent InvitrogenTM L3000150 FuGENE 6 transfection reagent Promega E2691 Maackia Amurensis Lectin I (MAL I) Vector Laboratories B-1315-2 Maackia Amurensis Lectin II (MAL II) Vector Laboratories B-1265-1 Erythrina Cristagalli Lectin (ECA) Vector Laboratories B-1145-5 Biotinylated Sambucus Nigra Lectin (SNA) Vector Laboratories B-1305-2 Sambucus Nigra Lectin (SNA) Vector Laboratories L-1300-5 Biotinylated Pan-specific Lectenz LectenzBio SK0502B Biotinylated α2,3-specific Lectenz LectenzBio SK2301B Dulbecco’s PBS + Ca2+/Mg2+ Capricorn scientific PBS-2A DMEM High Glucose with L-Glutamine Capricorn scientific CA DMEM-HA Opti-MEM + GlutaMAX GIBCO 51985–026 Penicillin-Streptomycin Thermo Fisher Scientific 15140122 Biotinylated LAMP1 (lysosomal membrane protein 1) Liu et al.17 N/A Biotinylated GPa (glycophorin A) Liu et al.13 N/A Fetal Bovine Serum Biowest S1520 Alexa Fluor647-conjugated streptavidin Invitrogen S21374 GFP-tagged CAS9PKBS Addgene Plasmid #68371 Oseltamivir Carboxylate Hoffman-La Roche Contact Roche SA sensors biolayer interferometry Sartorius 18–5020 Critical commercial assays Renilla luciferase assay system Promega E2810 (Continued on next page) iScience 28, 112549, June 20, 2025 e1 .. The human H3N2 recombinant influenza strains used in this paper represent the antigenic clusters identified since 1968 and were generated and described previously.40 They are 2:6 reassortants containing the 6 internal gene segments of strain A/Puerto Rico/ 8/1934(H1N1) and the HA and NA gene segments of: A/Bilthoven/16190/68 (HK68Hu− H3), A/Bilthoven/21793/72 (EN72Hu− H3), A/Bilthoven/1761/76 (VI75Hu− H3), A/Bilthoven/2271/76 (TX77Hu− H3), A/Netherlands/233/82-193N (BK79Hu− H3), A/Netherlands/620/89

    Recombinant:

    Article Title: Cell-Based Sialoglycan Arrays for directly comparing Influenza A virus Receptor Requirements for binding and infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat-anti-human IgG ThermoFisher A-11013 Anti-HA antibody MEDI produced in HEK293F cells This paper N/A Bacterial and virus strains A/Bilthoven/16190/68 (H3N2; HK68Hu− H3) Koel et al.40 N/A A/Bilthoven/21793/72 (H3N2; EN72 Hu− H3) Koel et al.40 N/A A/Bilthoven/1761/76 (H3N2; VI75 Hu− H3) Koel et al.40 N/A A/Bilthoven/2271/76 (H3N2; TX77 Hu− H3) Koel et al.40 N/A A/Netherlands/233/82-193N (H3N2; BK79 Hu− H3) Koel et al.40 N/A A/Netherlands/620/89 (H3N2; SI87 Hu− H3) Koel et al.40 N/A A/Netherlands/179/93 (H3N2; BE92 Hu− H3) Koel et al.40 N/A A/Netherlands/178/95-145K (H3N2; WU95 Hu− H3) Koel et al.40 N/A A/Netherlands/427/98 (H3N2; SY97 Hu− H3) Koel et al.40 N/A A/Netherlands/213/03 (H3N2; FU02 Hu− H3) Koel et al.40 N/A A/Perth/16/2009 (H3N2; PE09 Hu− H3) Koel et al.40 N/A A/duck/Hunan/795/2002(H5N1; HU02 Av− H5) Liu et al.17 N/A A/Netherlands/602/2009 (H1N1; NE09Hu− H1) Maines et al.57 N/A pRluc-53CB3/T7 van der Schaar et al.58 N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 transfection reagent InvitrogenTM 11668019 Lipofectamine 3000 transfection reagent InvitrogenTM L3000150 FuGENE 6 transfection reagent Promega E2691 Maackia Amurensis Lectin I (MAL I) Vector Laboratories B-1315-2 Maackia Amurensis Lectin II (MAL II) Vector Laboratories B-1265-1 Erythrina Cristagalli Lectin (ECA) Vector Laboratories B-1145-5 Biotinylated Sambucus Nigra Lectin (SNA) Vector Laboratories B-1305-2 Sambucus Nigra Lectin (SNA) Vector Laboratories L-1300-5 Biotinylated Pan-specific Lectenz LectenzBio SK0502B Biotinylated α2,3-specific Lectenz LectenzBio SK2301B Dulbecco’s PBS + Ca2+/Mg2+ Capricorn scientific PBS-2A DMEM High Glucose with L-Glutamine Capricorn scientific CA DMEM-HA Opti-MEM + GlutaMAX GIBCO 51985–026 Penicillin-Streptomycin Thermo Fisher Scientific 15140122 Biotinylated LAMP1 (lysosomal membrane protein 1) Liu et al.17 N/A Biotinylated GPa (glycophorin A) Liu et al.13 N/A Fetal Bovine Serum Biowest S1520 Alexa Fluor647-conjugated streptavidin Invitrogen S21374 GFP-tagged CAS9PKBS Addgene Plasmid #68371 Oseltamivir Carboxylate Hoffman-La Roche Contact Roche SA sensors biolayer interferometry Sartorius 18–5020 Critical commercial assays Renilla luciferase assay system Promega E2810 (Continued on next page) iScience 28, 112549, June 20, 2025 e1 .. The human H3N2 recombinant influenza strains used in this paper represent the antigenic clusters identified since 1968 and were generated and described previously.40 They are 2:6 reassortants containing the 6 internal gene segments of strain A/Puerto Rico/ 8/1934(H1N1) and the HA and NA gene segments of: A/Bilthoven/16190/68 (HK68Hu− H3), A/Bilthoven/21793/72 (EN72Hu− H3), A/Bilthoven/1761/76 (VI75Hu− H3), A/Bilthoven/2271/76 (TX77Hu− H3), A/Netherlands/233/82-193N (BK79Hu− H3), A/Netherlands/620/89

    Transfection:

    Article Title: Cell-Based Sialoglycan Arrays for directly comparing Influenza A virus Receptor Requirements for binding and infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat-anti-human IgG ThermoFisher A-11013 Anti-HA antibody MEDI produced in HEK293F cells This paper N/A Bacterial and virus strains A/Bilthoven/16190/68 (H3N2; HK68Hu− H3) Koel et al.40 N/A A/Bilthoven/21793/72 (H3N2; EN72 Hu− H3) Koel et al.40 N/A A/Bilthoven/1761/76 (H3N2; VI75 Hu− H3) Koel et al.40 N/A A/Bilthoven/2271/76 (H3N2; TX77 Hu− H3) Koel et al.40 N/A A/Netherlands/233/82-193N (H3N2; BK79 Hu− H3) Koel et al.40 N/A A/Netherlands/620/89 (H3N2; SI87 Hu− H3) Koel et al.40 N/A A/Netherlands/179/93 (H3N2; BE92 Hu− H3) Koel et al.40 N/A A/Netherlands/178/95-145K (H3N2; WU95 Hu− H3) Koel et al.40 N/A A/Netherlands/427/98 (H3N2; SY97 Hu− H3) Koel et al.40 N/A A/Netherlands/213/03 (H3N2; FU02 Hu− H3) Koel et al.40 N/A A/Perth/16/2009 (H3N2; PE09 Hu− H3) Koel et al.40 N/A A/duck/Hunan/795/2002(H5N1; HU02 Av− H5) Liu et al.17 N/A A/Netherlands/602/2009 (H1N1; NE09Hu− H1) Maines et al.57 N/A pRluc-53CB3/T7 van der Schaar et al.58 N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 transfection reagent InvitrogenTM 11668019 Lipofectamine 3000 transfection reagent InvitrogenTM L3000150 FuGENE 6 transfection reagent Promega E2691 Maackia Amurensis Lectin I (MAL I) Vector Laboratories B-1315-2 Maackia Amurensis Lectin II (MAL II) Vector Laboratories B-1265-1 Erythrina Cristagalli Lectin (ECA) Vector Laboratories B-1145-5 Biotinylated Sambucus Nigra Lectin (SNA) Vector Laboratories B-1305-2 Sambucus Nigra Lectin (SNA) Vector Laboratories L-1300-5 Biotinylated Pan-specific Lectenz LectenzBio SK0502B Biotinylated α2,3-specific Lectenz LectenzBio SK2301B Dulbecco’s PBS + Ca2+/Mg2+ Capricorn scientific PBS-2A DMEM High Glucose with L-Glutamine Capricorn scientific CA DMEM-HA Opti-MEM + GlutaMAX GIBCO 51985–026 Penicillin-Streptomycin Thermo Fisher Scientific 15140122 Biotinylated LAMP1 (lysosomal membrane protein 1) Liu et al.17 N/A Biotinylated GPa (glycophorin A) Liu et al.13 N/A Fetal Bovine Serum Biowest S1520 Alexa Fluor647-conjugated streptavidin Invitrogen S21374 GFP-tagged CAS9PKBS Addgene Plasmid #68371 Oseltamivir Carboxylate Hoffman-La Roche Contact Roche SA sensors biolayer interferometry Sartorius 18–5020 Critical commercial assays Renilla luciferase assay system Promega E2810 (Continued on next page) iScience 28, 112549, June 20, 2025 e1 .. The human H3N2 recombinant influenza strains used in this paper represent the antigenic clusters identified since 1968 and were generated and described previously.40 They are 2:6 reassortants containing the 6 internal gene segments of strain A/Puerto Rico/ 8/1934(H1N1) and the HA and NA gene segments of: A/Bilthoven/16190/68 (HK68Hu− H3), A/Bilthoven/21793/72 (EN72Hu− H3), A/Bilthoven/1761/76 (VI75Hu− H3), A/Bilthoven/2271/76 (TX77Hu− H3), A/Netherlands/233/82-193N (BK79Hu− H3), A/Netherlands/620/89

    Membrane:

    Article Title: Cell-Based Sialoglycan Arrays for directly comparing Influenza A virus Receptor Requirements for binding and infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat-anti-human IgG ThermoFisher A-11013 Anti-HA antibody MEDI produced in HEK293F cells This paper N/A Bacterial and virus strains A/Bilthoven/16190/68 (H3N2; HK68Hu− H3) Koel et al.40 N/A A/Bilthoven/21793/72 (H3N2; EN72 Hu− H3) Koel et al.40 N/A A/Bilthoven/1761/76 (H3N2; VI75 Hu− H3) Koel et al.40 N/A A/Bilthoven/2271/76 (H3N2; TX77 Hu− H3) Koel et al.40 N/A A/Netherlands/233/82-193N (H3N2; BK79 Hu− H3) Koel et al.40 N/A A/Netherlands/620/89 (H3N2; SI87 Hu− H3) Koel et al.40 N/A A/Netherlands/179/93 (H3N2; BE92 Hu− H3) Koel et al.40 N/A A/Netherlands/178/95-145K (H3N2; WU95 Hu− H3) Koel et al.40 N/A A/Netherlands/427/98 (H3N2; SY97 Hu− H3) Koel et al.40 N/A A/Netherlands/213/03 (H3N2; FU02 Hu− H3) Koel et al.40 N/A A/Perth/16/2009 (H3N2; PE09 Hu− H3) Koel et al.40 N/A A/duck/Hunan/795/2002(H5N1; HU02 Av− H5) Liu et al.17 N/A A/Netherlands/602/2009 (H1N1; NE09Hu− H1) Maines et al.57 N/A pRluc-53CB3/T7 van der Schaar et al.58 N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 transfection reagent InvitrogenTM 11668019 Lipofectamine 3000 transfection reagent InvitrogenTM L3000150 FuGENE 6 transfection reagent Promega E2691 Maackia Amurensis Lectin I (MAL I) Vector Laboratories B-1315-2 Maackia Amurensis Lectin II (MAL II) Vector Laboratories B-1265-1 Erythrina Cristagalli Lectin (ECA) Vector Laboratories B-1145-5 Biotinylated Sambucus Nigra Lectin (SNA) Vector Laboratories B-1305-2 Sambucus Nigra Lectin (SNA) Vector Laboratories L-1300-5 Biotinylated Pan-specific Lectenz LectenzBio SK0502B Biotinylated α2,3-specific Lectenz LectenzBio SK2301B Dulbecco’s PBS + Ca2+/Mg2+ Capricorn scientific PBS-2A DMEM High Glucose with L-Glutamine Capricorn scientific CA DMEM-HA Opti-MEM + GlutaMAX GIBCO 51985–026 Penicillin-Streptomycin Thermo Fisher Scientific 15140122 Biotinylated LAMP1 (lysosomal membrane protein 1) Liu et al.17 N/A Biotinylated GPa (glycophorin A) Liu et al.13 N/A Fetal Bovine Serum Biowest S1520 Alexa Fluor647-conjugated streptavidin Invitrogen S21374 GFP-tagged CAS9PKBS Addgene Plasmid #68371 Oseltamivir Carboxylate Hoffman-La Roche Contact Roche SA sensors biolayer interferometry Sartorius 18–5020 Critical commercial assays Renilla luciferase assay system Promega E2810 (Continued on next page) iScience 28, 112549, June 20, 2025 e1 .. The human H3N2 recombinant influenza strains used in this paper represent the antigenic clusters identified since 1968 and were generated and described previously.40 They are 2:6 reassortants containing the 6 internal gene segments of strain A/Puerto Rico/ 8/1934(H1N1) and the HA and NA gene segments of: A/Bilthoven/16190/68 (HK68Hu− H3), A/Bilthoven/21793/72 (EN72Hu− H3), A/Bilthoven/1761/76 (VI75Hu− H3), A/Bilthoven/2271/76 (TX77Hu− H3), A/Netherlands/233/82-193N (BK79Hu− H3), A/Netherlands/620/89

    Plasmid Preparation:

    Article Title: Cell-Based Sialoglycan Arrays for directly comparing Influenza A virus Receptor Requirements for binding and infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat-anti-human IgG ThermoFisher A-11013 Anti-HA antibody MEDI produced in HEK293F cells This paper N/A Bacterial and virus strains A/Bilthoven/16190/68 (H3N2; HK68Hu− H3) Koel et al.40 N/A A/Bilthoven/21793/72 (H3N2; EN72 Hu− H3) Koel et al.40 N/A A/Bilthoven/1761/76 (H3N2; VI75 Hu− H3) Koel et al.40 N/A A/Bilthoven/2271/76 (H3N2; TX77 Hu− H3) Koel et al.40 N/A A/Netherlands/233/82-193N (H3N2; BK79 Hu− H3) Koel et al.40 N/A A/Netherlands/620/89 (H3N2; SI87 Hu− H3) Koel et al.40 N/A A/Netherlands/179/93 (H3N2; BE92 Hu− H3) Koel et al.40 N/A A/Netherlands/178/95-145K (H3N2; WU95 Hu− H3) Koel et al.40 N/A A/Netherlands/427/98 (H3N2; SY97 Hu− H3) Koel et al.40 N/A A/Netherlands/213/03 (H3N2; FU02 Hu− H3) Koel et al.40 N/A A/Perth/16/2009 (H3N2; PE09 Hu− H3) Koel et al.40 N/A A/duck/Hunan/795/2002(H5N1; HU02 Av− H5) Liu et al.17 N/A A/Netherlands/602/2009 (H1N1; NE09Hu− H1) Maines et al.57 N/A pRluc-53CB3/T7 van der Schaar et al.58 N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 transfection reagent InvitrogenTM 11668019 Lipofectamine 3000 transfection reagent InvitrogenTM L3000150 FuGENE 6 transfection reagent Promega E2691 Maackia Amurensis Lectin I (MAL I) Vector Laboratories B-1315-2 Maackia Amurensis Lectin II (MAL II) Vector Laboratories B-1265-1 Erythrina Cristagalli Lectin (ECA) Vector Laboratories B-1145-5 Biotinylated Sambucus Nigra Lectin (SNA) Vector Laboratories B-1305-2 Sambucus Nigra Lectin (SNA) Vector Laboratories L-1300-5 Biotinylated Pan-specific Lectenz LectenzBio SK0502B Biotinylated α2,3-specific Lectenz LectenzBio SK2301B Dulbecco’s PBS + Ca2+/Mg2+ Capricorn scientific PBS-2A DMEM High Glucose with L-Glutamine Capricorn scientific CA DMEM-HA Opti-MEM + GlutaMAX GIBCO 51985–026 Penicillin-Streptomycin Thermo Fisher Scientific 15140122 Biotinylated LAMP1 (lysosomal membrane protein 1) Liu et al.17 N/A Biotinylated GPa (glycophorin A) Liu et al.13 N/A Fetal Bovine Serum Biowest S1520 Alexa Fluor647-conjugated streptavidin Invitrogen S21374 GFP-tagged CAS9PKBS Addgene Plasmid #68371 Oseltamivir Carboxylate Hoffman-La Roche Contact Roche SA sensors biolayer interferometry Sartorius 18–5020 Critical commercial assays Renilla luciferase assay system Promega E2810 (Continued on next page) iScience 28, 112549, June 20, 2025 e1 .. The human H3N2 recombinant influenza strains used in this paper represent the antigenic clusters identified since 1968 and were generated and described previously.40 They are 2:6 reassortants containing the 6 internal gene segments of strain A/Puerto Rico/ 8/1934(H1N1) and the HA and NA gene segments of: A/Bilthoven/16190/68 (HK68Hu− H3), A/Bilthoven/21793/72 (EN72Hu− H3), A/Bilthoven/1761/76 (VI75Hu− H3), A/Bilthoven/2271/76 (TX77Hu− H3), A/Netherlands/233/82-193N (BK79Hu− H3), A/Netherlands/620/89

    Luciferase:

    Article Title: Cell-Based Sialoglycan Arrays for directly comparing Influenza A virus Receptor Requirements for binding and infection
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488 goat-anti-human IgG ThermoFisher A-11013 Anti-HA antibody MEDI produced in HEK293F cells This paper N/A Bacterial and virus strains A/Bilthoven/16190/68 (H3N2; HK68Hu− H3) Koel et al.40 N/A A/Bilthoven/21793/72 (H3N2; EN72 Hu− H3) Koel et al.40 N/A A/Bilthoven/1761/76 (H3N2; VI75 Hu− H3) Koel et al.40 N/A A/Bilthoven/2271/76 (H3N2; TX77 Hu− H3) Koel et al.40 N/A A/Netherlands/233/82-193N (H3N2; BK79 Hu− H3) Koel et al.40 N/A A/Netherlands/620/89 (H3N2; SI87 Hu− H3) Koel et al.40 N/A A/Netherlands/179/93 (H3N2; BE92 Hu− H3) Koel et al.40 N/A A/Netherlands/178/95-145K (H3N2; WU95 Hu− H3) Koel et al.40 N/A A/Netherlands/427/98 (H3N2; SY97 Hu− H3) Koel et al.40 N/A A/Netherlands/213/03 (H3N2; FU02 Hu− H3) Koel et al.40 N/A A/Perth/16/2009 (H3N2; PE09 Hu− H3) Koel et al.40 N/A A/duck/Hunan/795/2002(H5N1; HU02 Av− H5) Liu et al.17 N/A A/Netherlands/602/2009 (H1N1; NE09Hu− H1) Maines et al.57 N/A pRluc-53CB3/T7 van der Schaar et al.58 N/A Chemicals, peptides, and recombinant proteins Lipofectamine 2000 transfection reagent InvitrogenTM 11668019 Lipofectamine 3000 transfection reagent InvitrogenTM L3000150 FuGENE 6 transfection reagent Promega E2691 Maackia Amurensis Lectin I (MAL I) Vector Laboratories B-1315-2 Maackia Amurensis Lectin II (MAL II) Vector Laboratories B-1265-1 Erythrina Cristagalli Lectin (ECA) Vector Laboratories B-1145-5 Biotinylated Sambucus Nigra Lectin (SNA) Vector Laboratories B-1305-2 Sambucus Nigra Lectin (SNA) Vector Laboratories L-1300-5 Biotinylated Pan-specific Lectenz LectenzBio SK0502B Biotinylated α2,3-specific Lectenz LectenzBio SK2301B Dulbecco’s PBS + Ca2+/Mg2+ Capricorn scientific PBS-2A DMEM High Glucose with L-Glutamine Capricorn scientific CA DMEM-HA Opti-MEM + GlutaMAX GIBCO 51985–026 Penicillin-Streptomycin Thermo Fisher Scientific 15140122 Biotinylated LAMP1 (lysosomal membrane protein 1) Liu et al.17 N/A Biotinylated GPa (glycophorin A) Liu et al.13 N/A Fetal Bovine Serum Biowest S1520 Alexa Fluor647-conjugated streptavidin Invitrogen S21374 GFP-tagged CAS9PKBS Addgene Plasmid #68371 Oseltamivir Carboxylate Hoffman-La Roche Contact Roche SA sensors biolayer interferometry Sartorius 18–5020 Critical commercial assays Renilla luciferase assay system Promega E2810 (Continued on next page) iScience 28, 112549, June 20, 2025 e1 .. The human H3N2 recombinant influenza strains used in this paper represent the antigenic clusters identified since 1968 and were generated and described previously.40 They are 2:6 reassortants containing the 6 internal gene segments of strain A/Puerto Rico/ 8/1934(H1N1) and the HA and NA gene segments of: A/Bilthoven/16190/68 (HK68Hu− H3), A/Bilthoven/21793/72 (EN72Hu− H3), A/Bilthoven/1761/76 (VI75Hu− H3), A/Bilthoven/2271/76 (TX77Hu− H3), A/Netherlands/233/82-193N (BK79Hu− H3), A/Netherlands/620/89

    Activity Assay:

    Article Title: Methods for manufacturing phthalocyanine dye conjugates and stable conjugates
    Article Snippet: .. In an exemplary additional study, the effect of Sambucus Nigra Lectin (SNA; also called Elderberry lectin, EBL) (Catalog No: L-1300, Vector Labs, Burlingame, CA) conjugated to IRDye 700DX was evaluated to assess its killing activity. ..



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    Lectin profiling of sialoglycans installed by transfection of individual specific STs on glycoconjugate-specific cells devoid of endogenous STs (A) Schematic illustration of CRISPR/Cas9-directed truncation of specific glycoconjugate classes in HEK ΔSia cells to produce sialyltransferase-depleted cells expressing only N-glycans (HEK ΔSia N), only O-glycans (HEK ΔSia O) or only GSLs (HEK ΔSia GSL). A sialoglycan-specific cell array is produced by transient introduction of a single ST including ST3Gal1 to ST3Gal6 or ST6Gal1 to reinstall ST-specific sialylation capacities. Glycan schemes display representative examples of non-sialylated glycans expressed in HEK ΔSia N cells (tri-antennary N-glycans), HEK ΔSia O cells (core 1 and 2 O-glycans) or HEK ΔSia GSL cells (glycosphingolipid core structures of ( neo )lacto-, ganglio-, and globo-series and Lac-Cer). Reported specificities of STs for either type I/II LN (Galβ1-3/4GlcNAc), type III LN (Galβ1-3GalNAc) or Lac-Cer (Galβ1-3Glc-Cer) are displayed. Not shown are ST3Gal3 and 6 which both act on type I/II LN. The cell-based array can be screened for virus infection (luciferase reporter assay) or virus binding (FACS analysis). In parallel, secreted glycoproteins (LAMP1 or Glycophorin A) carrying a biotinylation-tag (BAP-tag) can be transfected in the cell array to produce a specifically glycosylated protein library for binding analysis using biolayer interferometry (BLI). (B–D) Lectin binding to HEK WT cells (WT) and glycoconjugate-specific cell lines (color-coded) transfected with specific STs as indicated on radar plots. Empty vector-transfected cells (no ST) are taken along for detection of Sia-independent background binding. According to the manufacturer Pan-Lec binds to any terminal located Sia and 2-3-Lec binds to α2-3-linked terminal Sia. SNA binds to α2-6-linked Sia on Galβ1-4GlcNAc (type II LN). (E–H) Lectin binding, determined by BLI, to LAMP I glycoprotein expressed in the glyconjugate-specific cell lines (color-coded) transfected by specific STs. MAL I binds to α2-3-linked terminal Sia on type II LN and MAL II binds to α2-3-linked terminal Sia on type III LN. ECA binds to terminal type II LN. (I–L) Lectin binding, determined by BLI, to GPa glycoprotein expressed as described in (E–H). Data are represented as mean.

    Journal: iScience

    Article Title: Cell-based sialoglycan arrays for directly comparing influenza A virus receptor requirements for binding and infection

    doi: 10.1016/j.isci.2025.112549

    Figure Lengend Snippet: Lectin profiling of sialoglycans installed by transfection of individual specific STs on glycoconjugate-specific cells devoid of endogenous STs (A) Schematic illustration of CRISPR/Cas9-directed truncation of specific glycoconjugate classes in HEK ΔSia cells to produce sialyltransferase-depleted cells expressing only N-glycans (HEK ΔSia N), only O-glycans (HEK ΔSia O) or only GSLs (HEK ΔSia GSL). A sialoglycan-specific cell array is produced by transient introduction of a single ST including ST3Gal1 to ST3Gal6 or ST6Gal1 to reinstall ST-specific sialylation capacities. Glycan schemes display representative examples of non-sialylated glycans expressed in HEK ΔSia N cells (tri-antennary N-glycans), HEK ΔSia O cells (core 1 and 2 O-glycans) or HEK ΔSia GSL cells (glycosphingolipid core structures of ( neo )lacto-, ganglio-, and globo-series and Lac-Cer). Reported specificities of STs for either type I/II LN (Galβ1-3/4GlcNAc), type III LN (Galβ1-3GalNAc) or Lac-Cer (Galβ1-3Glc-Cer) are displayed. Not shown are ST3Gal3 and 6 which both act on type I/II LN. The cell-based array can be screened for virus infection (luciferase reporter assay) or virus binding (FACS analysis). In parallel, secreted glycoproteins (LAMP1 or Glycophorin A) carrying a biotinylation-tag (BAP-tag) can be transfected in the cell array to produce a specifically glycosylated protein library for binding analysis using biolayer interferometry (BLI). (B–D) Lectin binding to HEK WT cells (WT) and glycoconjugate-specific cell lines (color-coded) transfected with specific STs as indicated on radar plots. Empty vector-transfected cells (no ST) are taken along for detection of Sia-independent background binding. According to the manufacturer Pan-Lec binds to any terminal located Sia and 2-3-Lec binds to α2-3-linked terminal Sia. SNA binds to α2-6-linked Sia on Galβ1-4GlcNAc (type II LN). (E–H) Lectin binding, determined by BLI, to LAMP I glycoprotein expressed in the glyconjugate-specific cell lines (color-coded) transfected by specific STs. MAL I binds to α2-3-linked terminal Sia on type II LN and MAL II binds to α2-3-linked terminal Sia on type III LN. ECA binds to terminal type II LN. (I–L) Lectin binding, determined by BLI, to GPa glycoprotein expressed as described in (E–H). Data are represented as mean.

    Article Snippet: Sambucus Nigra Lectin (SNA) , Vector Laboratories , L-1300-5.

    Techniques: Transfection, CRISPR, Expressing, Produced, Glycoproteomics, Virus, Infection, Luciferase, Reporter Assay, Binding Assay, Plasmid Preparation